PicoPipet單細(xì)胞分離提取系統(tǒng)------捕獲懸浮狀態(tài)的細(xì)胞、顆粒,單細(xì)胞研究的最佳基礎(chǔ)工具。
日本NEPA GENE推出PicoPipet系統(tǒng)可以方便分離捕獲懸液中的單細(xì)胞和微粒。單細(xì)胞培養(yǎng),可最真實(shí)的獲得單克隆的細(xì)胞,用于純化、鑒定、及各種單細(xì)胞水平的檢測(cè)。基于單個(gè)細(xì)胞水平,后續(xù)采取單細(xì)胞全基因組擴(kuò)增、單細(xì)胞測(cè)序等技術(shù),用于揭示細(xì)胞群體差異和細(xì)胞進(jìn)化關(guān)系,用于檢測(cè)單細(xì)胞的具體突變來(lái)源及精準(zhǔn)的突變頻率,用于腫瘤發(fā)生機(jī)制及胚胎發(fā)育研究等。PicoPipet單細(xì)胞分離提取系統(tǒng)有不同的型號(hào)可選:MPP-300z、MPP-300、MPP-200A、MPP-200z、MPP-200B。
主要特點(diǎn):
- 整套系統(tǒng)由PicoPipet超微量吸液器和電動(dòng)控制器、Compass顯微鏡成像、Joystick顯微操縱器三大部分組成
- 成像系統(tǒng)大屏顯示、分辨率高,可全程監(jiān)測(cè)捕獲過(guò)程
- 操作簡(jiǎn)單,精確度高,定位目標(biāo)→吸取捕獲→轉(zhuǎn)移釋放,三步完成單細(xì)胞的分離提取
- 可搭配不同直徑的毛細(xì)管、應(yīng)用范圍廣
應(yīng)用范圍:
- 可分離捕獲各種非貼壁狀態(tài)的單細(xì)胞和微粒等
- 可分離捕獲細(xì)菌、酵母、霉菌孢子、藻類細(xì)胞、花粉、懸浮細(xì)胞、晶體、金屬粒子
- 可分離捕獲原生質(zhì)體、原生動(dòng)物單細(xì)胞、血液細(xì)胞、免疫細(xì)胞、卵細(xì)胞、各種懸液中單細(xì)胞及微粒
- 用于各種類型的單細(xì)胞分離培養(yǎng)、純化、檢測(cè);獲得單克隆細(xì)胞;用于單細(xì)胞基因擴(kuò)增(Single-cell
PCR),用于單細(xì)胞測(cè)序等
主要配置:
標(biāo)配項(xiàng)目
|
規(guī)格描述
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數(shù)量
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PicoPipet
&Controller
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電動(dòng)超微量吸液器(含控制器)
|
1
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Compass
|
顯微鏡及成像系統(tǒng)
|
1
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Joystick
Manipulater for Compass
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搖桿顯微操縱器
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1
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以下選配項(xiàng):
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Z-robo
for Compass
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Compass顯微鏡的Z軸自動(dòng)控制器
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|
Standard
glass tip
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直頭毛細(xì)玻璃管,可選Tip ID 15,30,50,75,100,125,150,175 or 200um,5個(gè)/包
|
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S-shaped
glass tip
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S型毛細(xì)玻璃管,可選Tip ID 30,50,75,100,125,150,175 or 200um,5個(gè)/包
|
|
PCR
cap holders
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PCR八連管支架 (用于單細(xì)胞PCR)
|
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日本NEPA GENE公司專業(yè)研發(fā)、生產(chǎn)細(xì)胞電轉(zhuǎn)染儀、電融合儀及單細(xì)胞分離提取系統(tǒng)等,其生產(chǎn)NEPA21系列電轉(zhuǎn)染儀、ECFG21高效細(xì)胞電融合與電穿孔儀及PicoPipet單細(xì)胞分離提取系統(tǒng)在研究領(lǐng)域中久負(fù)盛名,已被數(shù)百篇文獻(xiàn)引用,其中不乏高水平雜志的文章,如Nature、Cell、PNAS、Genes & Dvelopment等。
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